Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype.

نویسندگان

  • Karen C Jinneman
  • Ken J Yoshitomi
  • Stephen D Weagant
چکیده

A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H- uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. The PCR efficiencies were >/= 1.89, and as few as 6 CFU/reaction could be detected.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 69 10  شماره 

صفحات  -

تاریخ انتشار 2003